16S Metagenomics - Technology
Using the DNA used to construct the 16S subunit, we are able to identify the genus and the genus species in a sample. We use a metagenomic approach with the v4 16S region. This provides a genus and/or a genus-species identification for every single organism. The metagenomics approach allows companies to send in non-enriched samples, finished products, or various raw materials.
- Leveraging a proprietary 16S database, we are able to identify the genus of each bacteria present in a sample.
- Identify likely candidates for the organism of concern through operational taxonomic units (OTUs).
- Show Relative abundance of the genus of bacteria present in each sample.
Using 16S for microbiome applications allows for incredible resolution in food spoilage or plate microbiome applications. The 16s metagenomic analysis allows for some critical improvements over traditional subtyping methods.
16S vs. Traditional Methods
- Traditional methods suffer from two specific issues: volume of work, and media biases.
- The volume of work through subtyping a sample can be enormous. Each different organism needs to be isolated and then use a traditional biochemical identification. In an environmental sample from food production, this could mean 15-20 isolates!
- The other element is viable but not culturable. Traditional subtyping methods introduce a bias by only selecting organisms that can grow in the media used in isolation. 16S circumvents this issue by obtaining amplifying and identifying the 16S sequence in a neat sample (no isolation).
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