Neogen® is a certified satellite laboratory of BioTracking, the manufacturer of the BioPRYN, an early stage cattle pregnancy detection test. The BioPRYN test is a convenient, non-invasive alternative to palpation and ultrasound pregnancy evaluation that provides accurate and cost-efficient results from a blood sample. Using this test, early pregnancy detection is available for cattle, sheep, goat, and bison with results for pregnancy detection are generally available the day after receiving the samples (if received on Friday, results will be available the following Tuesday).
The BioPRYN test is an enzyme-linked immunosorbent assay (ELISA) that evaluates a serum or plasma sample for the presence of the pregnancy-specific protein B (PSPB). PSPB is only produced by the placenta of an animal with a growing fetus. PSPB concentrations can be accurately measured in blood within 28 days post-breeding (DPB) in adult cows, 25 DPB in heifers, 30 DPB in goat/sheep, and 40 DBP in bison. Animals must be at least 73 days post-calving.
For embryo transfer in cattle, blood can be taken at 28 days of embryo age (21 days after the transfer of a 7-day old embryo). When these protocols are followed, the test is 99% accurate when calling an animal "OPEN" and 95% accurate when a "PREGNANT" call is made.
Acceptable sample type:
2 mL (cc) of blood is requested for testing.
Please see blood collection assembly and use for assistance with tail bleeding cattle.
Samples must meet days post bred (DPB) requirements below:
Cows–28 DPB and 73 days post-calving
Embryo Transfer–Embryo age 28 days (21 days after transfer of a 7-day old embryo)
Requirements for Shipping:
PSPB is very stable in blood and samples can be shipped at ambient temperatures. Transit times should not exceed 3 days. Avoid freezing the samples.